Efficacy of novel nano-hydroxyapatite/polyurethane composite scaffolds with silver phosphate particles in chronic osteomyelitis.
PMID 31127361 · Journal of materials science. Materials in medicine · 2019 · DOI 10.1007/s10856-019-6261-7
Authors: Dongli Zhang; Wen Liu; Xiang-Dong Wu; Xiaoqiang He; Xiao Lin; Han Wang; Jidong Li; Jiaxing Jiang; Wei Huang
Abstract
Recently, chronic osteomyelitis is still a challenging surgical problem. Unfortunately, the traditional clinical method using bone cement loaded antibiotics is restricted due to its non-biodegradability and limited release of antibiotics. Hydroxyapatite is a good adsorbent with good biocompatibility, an ideal bone repair material, and can avert the requirement for the secondary surgical procedure of removal. In this study, nano-hydroxyapatite combined with a polyurethane containing 3% silver (Ag/n-HA/PU) was synthesized, and investigated for its efficacy of treating chronic bone infection with bone defects. To clarify its silver ions release characteristics, the concentration of the Ag+ in the elution was analyzed every day after in vitro deionized water immersion. A chronic osteomyelitis of tibia in rabbit model was established, and 70 New Zealand rabbits were divided into 4 groups, including the blank control group, nano-hydroxyapatite combined with polyurethane (n-HA/PU) implant group, 3% Ag/n-HA/PU group and 10% Ag/n-HA/PU group after debridement. Routine blood tests, radiography, Micro-CT, and histological staining were conducted at 4 days, 3, 6 and 12 weeks post-treatment. The results showed that the released silver from the 3% Ag/n-HA/PU and 10% Ag/n-HA/PU exhibited an initial burst release and followed by a slow controlled release up to 39 days and 42 days respectively. A good repair of bone defects, an appropriate rate of degradation of scaffolds and no significant toxicity were observed in the 3% Ag/n-HA/PU group, indicating the advantages of this novel synthetic scaffold to be a potential option for the treatment of chronic osteomyelitis. A novel nano-composite, nano-hydroxyapatite combined with a polyurethane containing 3% silver (Ag/n-HA/PU) provide controlled release of Ag+, illustrated by its abilities of biodegradation, antimicrobial activity, and favorable repair of bone defects in the treatment of chronic osteomyelitis.
Adsorption; Animals; Anti-Bacterial Agents; Biocompatible Materials; Bone Regeneration; Bone Substitutes; Chronic Disease; Disease Models, Animal; Durapatite; Male; Nanoparticles; Osteomyelitis; Phosphates; Polyurethanes; Rabbits; Silver Compounds; Staphylococcal Infections; Staphylococcus aureus; Tibia; Tissue Scaffolds; Tolonium Chloride; X-Ray Microtomography
Cite
Dongli Zhang; Wen Liu; Xiang-Dong Wu; Xiaoqiang He; Xiao Lin; Han Wang; Jidong Li; Jiaxing Jiang; Wei Huang. Efficacy of novel nano-hydroxyapatite/polyurethane composite scaffolds with silver phosphate particles in chronic osteomyelitis. Journal of materials science. Materials in medicine (2019). PMID 31127361. DOI 10.1007/s10856-019-6261-7.