Moving forward one step back at a time: reversibility during homologous recombination.
PMID 31123771 · Current genetics · 2019 · DOI 10.1007/s00294-019-00995-7
Authors: Aurèle Piazza; Wolf-Dietrich Heyer
Abstract
DNA double-strand breaks are genotoxic lesions whose repair can be templated off an intact DNA duplex through the conserved homologous recombination (HR) pathway. Because it mainly consists of a succession of non-covalent associations of molecules, HR is intrinsically reversible. Reversibility serves as an integral property of HR, exploited and tuned at various stages throughout the pathway with anti- and pro-recombinogenic consequences. Here, we focus on the reversibility of displacement loops (D-loops), a central DNA joint molecule intermediate whose dynamics and regulation have recently been physically probed in somatic S. cerevisiae cells. From homology search to repair completion, we discuss putative roles of D-loop reversibility in repair fidelity and outcome.
DNA Breaks, Double-Stranded; DNA Helicases; DNA-Binding Proteins; DNA-Directed DNA Polymerase; Endodeoxyribonucleases; Recombinational DNA Repair; Saccharomyces cerevisiae
Cite
Aurèle Piazza; Wolf-Dietrich Heyer. Moving forward one step back at a time: reversibility during homologous recombination. Current genetics (2019). PMID 31123771. DOI 10.1007/s00294-019-00995-7.