Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides.
PMID 31123731 · Chemical communications (Cambridge, England) · 2019 · DOI 10.1039/c8cc09648a
Authors: Takenori Shimo; Keisuke Tachibana; Yuki Kawawaki; Yuuka Watahiki; Taku Ishigaki; Yusuke Nakatsuji; Takashi Hara; Junji Kawakami; Satoshi Obika
Abstract
PAGE and UV melting analysis revealed that longer LNA-based splice-switching oligonucleotides (SSOs) formed secondary structures by themselves, reducing their effective concentration. To avoid such secondary structure formation, we introduced 7-deaza-2'-deoxyguanosine or 2'-deoxyinosine into the SSOs. These modified SSOs, with fewer secondary structures, showed higher exon skipping activities.
Deoxyguanosine; Exons; Inosine; Nucleic Acid Conformation; Oligonucleotides; Oxidation-Reduction
Cite
Takenori Shimo; Keisuke Tachibana; Yuki Kawawaki; Yuuka Watahiki; Taku Ishigaki; Yusuke Nakatsuji; Takashi Hara; Junji Kawakami; Satoshi Obika. Enhancement of exon skipping activity by reduction in the secondary structure content of LNA-based splice-switching oligonucleotides. Chemical communications (Cambridge, England) (2019). PMID 31123731. DOI 10.1039/c8cc09648a.