Kinetics of carbon monoxide binding to fully and partially reduced human hemoglobin valency hybrids.
PMID 23948 · European journal of biochemistry · 1978 · DOI 10.1111/j.1432-1033.1978.tb12096.x
Authors: H S Rollema; A Raap; S H de Bruin
Abstract
The kinetics of carbon monoxide binding following fast reduction of the valency hybrids alpha2+betaCO2 and alphaCO2beta+2 by hydrated electrons have been studied at different degrees of reduction. The results show that at pH 6.0 and 7.0 reduction of one heme group yields a species which reacts fast with carbon monoxide (rate constant of the order of 10(6) M-1S-1). At pH 6.0 the intermediates alphaCO2beta2 and alpha2betaCO2 bind carbon monoxide with a rate characteristic of the T state. At pH 7.0 alphaCO2beta2 is for the greater part in the T state, while in the case of alpha2betaCO2 the R and the T state are about equally populated.
Carbon Monoxide; Chemical Phenomena; Chemistry; Hemoglobins; Humans; Hydrogen-Ion Concentration; Kinetics; Macromolecular Substances
Cite
H S Rollema; A Raap; S H de Bruin. Kinetics of carbon monoxide binding to fully and partially reduced human hemoglobin valency hybrids. European journal of biochemistry (1978). PMID 23948. DOI 10.1111/j.1432-1033.1978.tb12096.x.