Flow injection chemiluminescence determination of naphazoline hydrochloride in pharmaceuticals.
PMID 23463582 · Luminescence : the journal of biological and chemical luminescence · 2014 · DOI 10.1002/bio.2500
Authors: Mortaza Iranifam; Mohammad H Sorouraddin
Abstract
A simple and sensitive flow injection chemiluminescence (FI-CL) method was developed for the determination of naphazoline hydrochloride (NPZ). The method is based on the enhancing effect of NPZ on the weak CL signal from the reaction of KIO4 with H2 O2 . Experimental parameters that affected the CL signal, including the pH of the KIO4 solution, concentrations of KIO4 , H2 O2 and disodium-EDTA and flow rate were optimized. Under the optimum conditions, the increment of CL intensity was linearly proportional to the concentration of NPZ in the range 5.0 × 10(-6) to 70 × 10(-6) mol/L. The detection limit was 1.0 × 10(-6) mol/L and the relative standard deviation for 50 × 10(-6) mol/L NPZ solution was 2.8% (n = 11). In addition, a high throughput of 120 samples/h was achieved. The utility of this method was demonstrated by determining NPZ in pharmaceuticals.
Flow Injection Analysis; Hydrogen Peroxide; Hydrogen-Ion Concentration; Luminescence; Naphazoline
Cite
Mortaza Iranifam; Mohammad H Sorouraddin. Flow injection chemiluminescence determination of naphazoline hydrochloride in pharmaceuticals. Luminescence : the journal of biological and chemical luminescence (2014). PMID 23463582. DOI 10.1002/bio.2500.