Veronaea botryosa: molecular identification with amplified fragment length polymorphism (AFLP) and in vitro antifungal susceptibility.
PMID 23463524 · Mycopathologia · 2013 · DOI 10.1007/s11046-013-9631-6
Authors: Hamid Badali; Seyed Amir Yazdanparast; Alexandro Bonifaz; Bita Mousavi; G Sybren de Hoog; Corné H W Klaassen; Jacques F Meis
Abstract
Inter- and intraspecific genomic variability of 18 isolates of Veronaea botryosa originating from clinical and environmental sources was studied using amplified fragment length polymorphism (AFLP). The species was originally described from the environment, but several severe cases of disseminated infection in apparently healthy individuals have been reported worldwide. All tested strains of V. botryosa, identified on the basis of sequencing and phenotypic and physiological criteria prior to our study, were confirmed by AFLP analysis, yielding a clear separation of V. botryosa as a rather homogeneous group from related species. In vitro antifungal susceptibility testing resulted in MIC90s across all strains in increasing order posaconazole (0.25 μg/ml), itraconazole (1 μg/ml), voriconazole (4 μg/ml), terbinafine (4 μg/ml), caspofungin (8 μg/ml), anidulafungin (8 μg/ml), isavuconazole (16 μg/ml), amphotericin B (16 μg/ml), and fluconazole (32 μg/ml). Overall, the isolates showed a uniform pattern of low MICs of itraconazole and posaconazole, but high MICs for remaining agents. The echinocandins (caspofungin and anidulafungin) had no activity against V. botryosa. There was no statistically significant difference between susceptibilities of environmental (n = 11) and clinical (n = 7) isolates of V. botryosa (P > 0.05).
Adolescent; Adult; Aged; Aged, 80 and over; Amplified Fragment Length Polymorphism Analysis; Antifungal Agents; Ascomycota; Child; Environmental Microbiology; Female; Humans; Male; Microbial Sensitivity Tests; Middle Aged; Mycological Typing Techniques; Mycoses; Young Adult
Cite
Hamid Badali; Seyed Amir Yazdanparast; Alexandro Bonifaz; Bita Mousavi; G Sybren de Hoog; Corné H W Klaassen; Jacques F Meis. Veronaea botryosa: molecular identification with amplified fragment length polymorphism (AFLP) and in vitro antifungal susceptibility. Mycopathologia (2013). PMID 23463524. DOI 10.1007/s11046-013-9631-6.