Changes in membrane fatty acid composition of Pseudomonas aeruginosa in response to UV-C radiations.
PMID 23463516 · Current microbiology · 2013 · DOI 10.1007/s00284-013-0342-5
Authors: Salma Kloula Ben Ghorbal; Abdelwaheb Chatti; Mohamed Marwan Sethom; Lobna Maalej; Mouadh Mihoub; Sana Kefacha; Moncef Feki; Ahmed Landoulsi; Abdennaceur Hassen
Abstract
The changes in lipid composition enable the micro-organisms to maintain membrane functions in the face of environmental fluctuations. The relationship between membrane fatty acid composition and UV-C stress was determined for mid-exponential phase and stationary phase Pseudomonas aeruginosa. The total lipids were obtained by dichloromethane/methanol (3:1) and were quantified by GC. The TLC analysis of phospholipids showed the presence of three major fractions phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin. Significant modifications, as manifested by an increase of UFA, were obtained. Interestingly, this microorganism showed a remarkable capacity for recovery from the stressful effects of UV-C.
Cell Membrane; Chromatography, Gas; Chromatography, Thin Layer; Fatty Acids; Pseudomonas aeruginosa; Stress, Physiological; Ultraviolet Rays
Cite
Salma Kloula Ben Ghorbal; Abdelwaheb Chatti; Mohamed Marwan Sethom; Lobna Maalej; Mouadh Mihoub; Sana Kefacha; Moncef Feki; Ahmed Landoulsi; Abdennaceur Hassen. Changes in membrane fatty acid composition of Pseudomonas aeruginosa in response to UV-C radiations. Current microbiology (2013). PMID 23463516. DOI 10.1007/s00284-013-0342-5.