Isolation and identification of environmental mycobacteria in the waters of a hemodialysis center.
PMID 23463515 · Current microbiology · 2013 · DOI 10.1007/s00284-013-0341-6
Authors: Flávio Garcia Sartori; Luís Fernando Leandro; Lilian Bueno Montanari; Maria Gorete Mendes de Souza; Regina Helena Pires; Daisy Nakamura Sato; Clarice Queico Fujimura Leite; Karina de Andrade Prince; Carlos Henrique Gomes Martins
Abstract
The use of poorly treated water during hemodialysis may lead to contamination with nontuberculous mycobacteria (NTM). This study aimed to isolate and identify NTM species in the water of a Brazilian hemodialysis center. We collected 210 samples of water from the hydric system of the unit (post-osmosis system, hemodialysis rooms, reuse system, and hemodialysis equipment) and from the municipal supply network; we isolated the NTM by a classic microbiological technique and identified them by the PCR restriction enzyme pattern of the hsp65 gene (PRA). Fifty-one (24.3 %) of the collected samples tested positive for NTM; both the municipal supply network (2 samples, 3.2 %) and the hydric system of the hemodialysis center (49 samples, 96.1 %) contained NTM. We isolated and identified potentially pathogenic bacteria such as Mycobacterium lentiflavum (59.0 %) and M. kansasii (5.0 %), as well as rarely pathogenic bacteria like M. gordonae (24.0 %), M. gastri (8.0 %), and M. szulgai (4.0 %). The ability of NTM to cause diseases is well documented in the literature. Therefore, the identification of NTM in the water of a Brazilian hemodialysis center calls for more effective water disinfection procedures in this unit.
Bacterial Proteins; Bacteriological Techniques; Brazil; Chaperonin 60; Disinfection; Genotype; Hemodialysis Units, Hospital; Infection Control; Molecular Diagnostic Techniques; Nontuberculous Mycobacteria; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Water Microbiology
Cite
Flávio Garcia Sartori; Luís Fernando Leandro; Lilian Bueno Montanari; Maria Gorete Mendes de Souza; Regina Helena Pires; Daisy Nakamura Sato; Clarice Queico Fujimura Leite; Karina de Andrade Prince; Carlos Henrique Gomes Martins. Isolation and identification of environmental mycobacteria in the waters of a hemodialysis center. Current microbiology (2013). PMID 23463515. DOI 10.1007/s00284-013-0341-6.