Passive administration of purified secretory IgA from human colostrum induces protection against Mycobacterium tuberculosis in a murine model of progressive pulmonary infection.
PMID 23458564 · BMC immunology · 2013 · DOI 10.1186/1471-2172-14-S1-S3
Authors: Nadine Alvarez; Oscar Otero; Frank Camacho; Reinier Borrero; Yanely Tirado; Alina Puig; Alicia Aguilar; Cesar Rivas; Axel Cervantes; Gustavo Falero-Díaz; Armando Cádiz; María E Sarmiento; Mohd Nor Norazmi; Rogelio Hernández-Pando; Armando Acosta
Abstract
Immunoglobulin A is the most abundant isotype in secretions from mucosal surfaces of the gastrointestinal, respiratory and genitourinary tracts and in external secretions such as colostrum, breast milk, tears and saliva. The high concentration of human secretory IgA (hsIgA) in human colostrum strongly suggests that it should play an important role in the passive immune protection against gastrointestinal and respiratory infections. Human secretory IgA was purified from colostrum. The reactivity of hsIgA against mycobacterial antigens and its protective capacity against mycobacterial infection was evaluated. The passive administration of hsIgA reduces the pneumonic area before challenge with M. tuberculosis. The intratracheal administration of M. tuberculosis preincubated with hsIgA to mice greatly reduced the bacterial load in the lungs and diminished lung tissue injury. HsIgA purified from colostrum protects against M. tuberculosis infection in an experimental mouse model.
Animals; Antigens, Bacterial; Bacterial Load; Colostrum; Disease Models, Animal; Humans; Immunization, Passive; Immunoglobulin A, Secretory; Mice; Mice, Inbred BALB C; Mycobacterium tuberculosis; Tuberculosis, Pulmonary
Cite
Nadine Alvarez; Oscar Otero; Frank Camacho; Reinier Borrero; Yanely Tirado; Alina Puig; Alicia Aguilar; Cesar Rivas; Axel Cervantes; Gustavo Falero-Díaz; Armando Cádiz; María E Sarmiento; Mohd Nor Norazmi; Rogelio Hernández-Pando; Armando Acosta. Passive administration of purified secretory IgA from human colostrum induces protection against Mycobacterium tuberculosis in a murine model of progressive pulmonary infection. BMC immunology (2013). PMID 23458564. DOI 10.1186/1471-2172-14-S1-S3.