The two dimensional structure of tetrazolium-mucopolysaccharide complexes deduced from reactivity studies.
PMID 23398 · The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 1978 · DOI 10.1177/26.1.23398
Authors: V A DeBari; M A Needle
Abstract
The formation of complexes between mucopolysaccharides and tetrazolium salts has been studied both by turbidimetry and nephelometry. The technique of laser nephelometry allows the detection of colloidal aggregates in systems which may, by turbidimetric methods, be ambiguous. The results indicate that binding of tetrazolium salts to polyanions can result in soluble as well as insoluble complexes; the monotetrazolium salts form soluble complexes and the ditetrazolium compounds form insoluble complexes. The insoluble complexes are stable at relatively low pH, and are disrupted during reduction to the formazan. Complex formation is decreased at high ratios of heparin to tetrazolium, and divalent cations, even at high concentration, do not precipitate mucopolysaccharides. It is concluded that stable ionic interactions with spatial charge separation are responsible for the cross linking of the mucopolysaccharides and the formaiton of large insoluble aggregates.
Chemical Phenomena; Chemistry; Chondroitin; Chondroitin Sulfates; Dextrans; Heparin; Hyaluronic Acid; Hydrogen-Ion Concentration; Nephelometry and Turbidimetry; Solubility; Tetrazolium Salts
Cite
V A DeBari; M A Needle. The two dimensional structure of tetrazolium-mucopolysaccharide complexes deduced from reactivity studies. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (1978). PMID 23398. DOI 10.1177/26.1.23398.