Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats.
PMID 20802452 · Neuro endocrinology letters · 2010
Authors: Nancy Cardoso; Matías Pandolfi; Osvaldo Ponzo; Silvia Carbone; Berta Szwarcfarb; Pablo Scacchi; Roxana Reynoso
Abstract
The aim of present paper was to study the probable role of glutamic acid (GLU) as a mediator of bisphenol A (BPA) effect at the hypothalamic level and its effects on the reproductive axis of prepubertal male rats. Mated Wistar rats were treated with either 0.1% ethanol (control group, n=10) or BPA (BPA group, n=10) in their drinking water until their offspring were weaned at the age of 21 days. The estimated average dose of exposure to dams was approximately 2.5 mg/kg body weight/day of BPA. At the prepubertal stage (35 days of age), the male rats were sacrificed and Gn-RH and glutamic acid (GLU) release, an amino acid involved in Gn-RH secretion, were measured in hypothalamic samples containing medio basal and anterior preoptic area (MBH-APOA), by RIA and HPLC respectively. LH, FSH serum levels were measured by RIA and testosterone by EQLIA. Gn-RH and GLU release decreased significantly in animals exposed to BPA (p<0.001, p<0.01). LH, FSH and testosterone serum levels were also decreased by treatment (p<0.0001). Present results provide evidence that BPA may act at the hypothalamic level to decrease GLU release which in turn may modify Gn-RH secretion altering the normal function of the axis.
Animals; Benzhydryl Compounds; Chromatography, High Pressure Liquid; Female; Follicle Stimulating Hormone; Glutamic Acid; Gonadotropin-Releasing Hormone; Hypothalamus; Luteinizing Hormone; Male; Phenols; Pregnancy; Prenatal Exposure Delayed Effects; Radioimmunoassay; Rats; Rats, Wistar; Sexual Maturation; Testosterone; Bisphenol A Compounds
Cite
Nancy Cardoso; Matías Pandolfi; Osvaldo Ponzo; Silvia Carbone; Berta Szwarcfarb; Pablo Scacchi; Roxana Reynoso. Evidence to suggest glutamic acid involvement in Bisphenol A effect at the hypothalamic level in prepubertal male rats. Neuro endocrinology letters (2010). PMID 20802452.