Proteomics for the elucidation of cold adaptation mechanisms in Listeria monocytogenes.
PMID 20620249 · Journal of proteomics · 2010 · DOI 10.1016/j.jprot.2010.06.011
Authors: Giuseppina Cacace; Maria F Mazzeo; Alida Sorrentino; Valentina Spada; Antonio Malorni; Rosa A Siciliano
Abstract
Listeria monocytogenes, one of the major food-related pathogens, is the aetiological agent of listeriosis, a potentially life-threatening illness. It is able to survive in hostile environments and stress conditions such as those encountered in food-processing technologies (high salt concentration, wide range of pH and temperature, low water availability) and it also thrives at temperatures ranging from -0.4 to 45 °C. In this study, expression proteomics was applied to gain insight into key cellular events that allow L. monocytogenes to survive and multiply even at refrigeration temperatures. Interestingly, we observed that the adaptation processes mainly affect biochemical pathways related to protein synthesis and folding, nutrient uptake and oxidative stress. Furthermore, proteins implicated in metabolic pathways for energy production, such as glycolysis and Pta-AckA pathway, were present to a higher level in the cells grown at 4 °C. This suggests that, on the whole, cells exhibit an enhanced demand for energy to sustain cold growth. Proteomics may represent a key tool in deciphering specific mechanisms underlying cold adaptation response and, more widely, cell machinery.
Adaptation, Physiological; Cold Temperature; Gene Expression Profiling; Listeria monocytogenes; Metabolic Networks and Pathways; Protein Folding; Proteomics
Cite
Giuseppina Cacace; Maria F Mazzeo; Alida Sorrentino; Valentina Spada; Antonio Malorni; Rosa A Siciliano. Proteomics for the elucidation of cold adaptation mechanisms in Listeria monocytogenes. Journal of proteomics (2010). PMID 20620249. DOI 10.1016/j.jprot.2010.06.011.