[Study on the anti-NTHi infection of Hap recombinant protein in vivo].
PMID 20619085 · Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology · 2010
Authors: Wan-yi Li; Bao-ning Wang; Feng-qiong Zuo; Wei Zeng; Feng Feng; Yu Kuang; Zhong-hua Jiang; Ming-yuan Li
Abstract
To observe the immune effect of Hap recombinant protein on murine model of bronchopneumonia infected with NTHi, and explore the mechanism about the anti-NTHi infection. The C57BL/6 mice intranasally immunized with purified Hap recombinant protein and CT-B were challenged by NTHi encased in agar beads. The immunifaction of anti-infection was observed through encocyte counting of BALF, bacteria detection of lung and the pathologyical change of lung tissue. In the challenge with NTHi experiment, the inflammatory exudation of the infected murine and pathological change of lung tissue was relieved by combined immunization of Hap recombinant protein and CT-B, and quantity of NTHi in lung of the infected murine was reduced obviously. The Hap recombinant protein also had good ability of anti-NTHi infection in the murine model of NTHi bronchopneumonia. This study could offer the oretical and experimental basis for development of new vaccine against NTHi.
Animals; Antibodies, Bacterial; Bacterial Outer Membrane Proteins; Disease Models, Animal; Female; Haemophilus Infections; Haemophilus influenzae; Humans; Immunization; Lung; Mice; Mice, Inbred C57BL; Random Allocation; Serine Endopeptidases
Cite
Wan-yi Li; Bao-ning Wang; Feng-qiong Zuo; Wei Zeng; Feng Feng; Yu Kuang; Zhong-hua Jiang; Ming-yuan Li. [Study on the anti-NTHi infection of Hap recombinant protein in vivo]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2010). PMID 20619085.