Hydroxylation of aromatic compounds by reduced nicotinamide-adenine dinucleotide and phenazine methosulphate requires hydrogen peroxide and hydroxyl radicals, but not superoxide.
PMID 201248 · The Biochemical journal · 1977 · DOI 10.1042/bj1670317
Authors: B Halliwell
Abstract
1. A mixture of NADH and phenazine methosulphate hydroxylates aromatic compounds at acidic pH values. 2. Hydroxylation is inhibited by catalase and by scavengers of the hydroxyl radical (-OH) but not by superoxide dismutase. 3. It is concluded that neither O2 leads to nor HO2- is sufficiently reactive to hydroxylate aromatic rings.
Hydrogen Peroxide; Hydroxybenzoates; Hydroxylation; Methylphenazonium Methosulfate; NAD; Phenazines; Superoxides
Cite
B Halliwell. Hydroxylation of aromatic compounds by reduced nicotinamide-adenine dinucleotide and phenazine methosulphate requires hydrogen peroxide and hydroxyl radicals, but not superoxide. The Biochemical journal (1977). PMID 201248. DOI 10.1042/bj1670317.