Tissue-cultured head and neck tumors: their use in in vitro assays of immune response.
PMID 196378 · Transactions. Section on Otolaryngology. American Academy of Ophthalmology and Otolaryngology · 1977
Authors: J C Aust; D Rabuzzi; G Reed
Abstract
Samples of 35 tumors from the head and neck region (25 squamous cell, 2 basal cell, 5 parotid, 3 melanoma, and 1 lymphosarcoma) were cultured after dispersement with either trypsin or collagenase treatment. Growth was established in 14 (40%). Cultured tumor cells were then used as target cells in in vitro assays of patients' cellular and humoral immunity to their own or similar tumors. Preliminary data suggest this may be a reliable method of monitoring responses in patients receiving immunotherapy for head and neck malignancies.
Adenocarcinoma; Basal Cell Carcinoma; Carcinoma, Squamous Cell; Culture Techniques; Head and Neck Neoplasms; Humans; Immunity; Immunity, Cellular; Lymphoma, Non-Hodgkin; Melanoma; Microbial Collagenase; Trypsin
Cite
J C Aust; D Rabuzzi; G Reed. Tissue-cultured head and neck tumors: their use in in vitro assays of immune response. Transactions. Section on Otolaryngology. American Academy of Ophthalmology and Otolaryngology (1977). PMID 196378.