Determination of the solution conformation of adenosein 2':3'-monophosphate by nuclear magnetic resonance with lanthanide probes.
PMID 192552 · European journal of biochemistry · 1977 · DOI 10.1111/j.1432-1033.1977.tb11398.x
Authors: G V Fazakerley; M A Wolfe
Abstract
The aqueous solution conformation of adenosine 2':3'-monophosphate at pH 2.5 has been determined by a nuclear magnetic resonance method utilizing lanthanide ions as shift and relaxation probes. The ribose conformation is best described as a rapid equilibrium of 2'-endo(3'-exo) and 3'-endo(2'-exo) conformations in a ratio of approximately 2 to 1. The orientation of the base relative to ribose is restricted to a narrow range about chiCN=-70 degrees.
Cyclic AMP; Isomerism; Magnetic Resonance Spectroscopy; Metals, Rare Earth; Molecular Conformation
Cite
G V Fazakerley; M A Wolfe. Determination of the solution conformation of adenosein 2':3'-monophosphate by nuclear magnetic resonance with lanthanide probes. European journal of biochemistry (1977). PMID 192552. DOI 10.1111/j.1432-1033.1977.tb11398.x.