[Isolation and purification of a preparation possessing leukocytosis-stimulating properties from pertussis bacteria].
PMID 190830 · Zhurnal mikrobiologii, epidemiologii i immunobiologii · 1977
Authors: N P Trofimova; A A Demina; Iu A Igonina; M S Dolomanova; G I Syresina
Abstract
A homogeneous protein LSF-2 preparation was extracted from the cultural fluid of Bordetella pertussis strains of the 1.0.3 serological type by means of precipitation with ammonium sulphate and electrofocussing; this preparation proved to produce a marked leukocytosis-stimulating and a weak toxic action of delayed type in experiments on animals. Intraperitoneal administration of 5 mug of the LSF-2 preparation caused a rise of leukocytosis in mice to 100,000 cells per 1 mm3, a delay in the gain in weight beginning from the 3rd day of the administration and a late death of the animals in 5% of cases. The LSF-2 preparation protected the mice in infection with a virulent pertussis strain No. 18323 in the amount of from 12 to 91%, depending on the immunizing dose; its ImD50 was equal to 2.0 -2.4 mug of protein. The results of investigations carried out permitted to assess the role of this substance in the formation of specific immunity in pertussis infection.
Animals; Antigens, Bacterial; Bacterial Proteins; Bacterial Toxins; Body Weight; Bordetella pertussis; Leukocytosis; Mice; Pertussis Vaccine; Vaccination; Whooping Cough
Cite
N P Trofimova; A A Demina; Iu A Igonina; M S Dolomanova; G I Syresina. [Isolation and purification of a preparation possessing leukocytosis-stimulating properties from pertussis bacteria]. Zhurnal mikrobiologii, epidemiologii i immunobiologii (1977). PMID 190830.