[Effect of glycogen on calcium of donor liver during ischemia-reperfusion period].
PMID 11856494 · Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2002
Authors: Yu Wang; Fuzhou Tian; Lijun Tang; Darong Huang; Xiaojun Li; Zhiliang Yin
Abstract
To evaluate the effect of glycogen on calcium concentration of rabbit donor liver during ischemia-reperfusion period. Donor group (n=21) was divided into 3 subgroups randomly: Group A (n=7): fasting for 24 hours before harvesting; Group B (n=7): normal laboratory chew; Group C (n=7): normal laboratory chew plus glucose supplement intravenously. Based on the self-created animal model for ischemia-reperfusion, the levels of glycogen content, ATP level, viability of Ca(2+)ATPase and plasmic free Ca(2+) concentration ([Ca(2+)]i) of liver tissue were measured. Before cold preservation, there was a significant difference of glycogen content among the three groups at all time points except at the end of rewarming period. ATP level and Ca(2+)ATPase viability were significantly higher in group C than in other two groups. But the plasmic free Ca(2+) concentration was lower in groups with higher glycogen content. Donor liver with high glycogen content can provide relatively sufficient ATP, maintain better Ca(2+)ATPase viability and prevent plasmic free Ca(2+) concentration overloading. This maybe an important mechanism for glycogen to ameliorate ischemia-reperfusion injury to the donor livers.
Adenosine Triphosphate; Animals; Calcium; Calcium-Transporting ATPases; Cytosol; Female; Glycogen; Liver Diseases; Liver Transplantation; Male; Models, Animal; Rabbits; Reperfusion Injury
Cite
Yu Wang; Fuzhou Tian; Lijun Tang; Darong Huang; Xiaojun Li; Zhiliang Yin. [Effect of glycogen on calcium of donor liver during ischemia-reperfusion period]. Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (2002). PMID 11856494.