Serine proteinase inhibitor 9 can be recognized by cytotoxic T lymphocytes of epithelial cancer patients.
PMID 11856484 · Japanese journal of cancer research : Gann · 2002 · DOI 10.1111/j.1349-7006.2002.tb01259.x
Authors: Koji Tanaka; Nanae Harashima; Fumihiko Niiya; Yoshiaki Miyagi; Naoya Hida; Mika Ochi; Nobue Imai; Mamoru Harada; Kyogo Itoh; Shigeki Shichijo
Abstract
Serine proteinase inhibitor 9 (PI-9) inhibits granzyme B-mediated apoptosis and interleukin-1beta-converting enzyme activity. In this study, we report that the PI-9 gene encodes antigenic epitopes recognized by the HLA-A24-restricted and tumor-reactive cytotoxic T lymphocytes (CTLs) of epithelial cancer patients. Screening of an autologous cDNA library using a CTL line recognizing HLA-A24+ tumor cells resulted in the isolation of a cDNA, which had an identical coding region to the previously described PI-9 genes. PI-9 gene was expressed in approximately three-fourths of epithelial cancer cell lines and all leukemic cell lines tested. It was also expressed in normal peripheral blood mononuclear cells (PBMCs), but not in a normal fibroblast cell line. CTL sublines contained T cells capable of recognizing the PI-9(292-300) and PI-9(348-356) peptides among 13 different peptides having the HLA-A24 binding motifs. These two peptides were recognized by the CTL line in a dose-dependent and HLA class-I-restricted manner, and also possessed the ability to induce HLA class I-restricted and tumor-reactive CTLs in PBMCs from HLA-A24+ cancer patients. These results demonstrate that PI-9 is recognized by HLA class I-restricted and tumor-reactive CTLs of epithelial cancer patients.
Base Sequence; Cloning, Molecular; Epitopes, T-Lymphocyte; HLA-A Antigens; HLA-A24 Antigen; Humans; Molecular Sequence Data; Neoplasms, Glandular and Epithelial; Serpins; T-Lymphocytes, Cytotoxic; Tumor Cells, Cultured
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Koji Tanaka; Nanae Harashima; Fumihiko Niiya; Yoshiaki Miyagi; Naoya Hida; Mika Ochi; Nobue Imai; Mamoru Harada; Kyogo Itoh; Shigeki Shichijo. Serine proteinase inhibitor 9 can be recognized by cytotoxic T lymphocytes of epithelial cancer patients. Japanese journal of cancer research : Gann (2002). PMID 11856484. DOI 10.1111/j.1349-7006.2002.tb01259.x.