Not infrequent K-ras mutations in depressed-type early colorectal carcinomas larger than 10 mm.
PMID 11856481 · Japanese journal of cancer research : Gann · 2002 · DOI 10.1111/j.1349-7006.2002.tb01256.x
Authors: Shinsuke Kazama; Yoichi Ajioka; Hidenobu Watanabe; Toshiaki Watanabe; Hirokazu Nagawa
Abstract
The aim of this study was to elucidate whether K-ras (codons 12 and 13) mutations occur in depressed-type early colorectal carcinomas (DECas) larger than 10 mm in size. Thirty-four cases of DECas including 27 larger than 10 mm were examined for K-ras mutations by means of microdissection, PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism), and direct sequencing. Although K-ras mutation was infrequent (1/7, 14%) in small (less than 10 mm) DECas, 16/27 (59%) and 17/27 (63%) of DECas larger than 10 mm revealed codon 12, or either codon 12 or 13 mutations, respectively. None of the evaluated pathological factors except size showed a correlation with K-ras mutation. These data indicate that although K-ras mutation could not be involved in the early stage of the progression of DECas, it might play a role at a later stage when the tumor size is over 10 mm.
Colorectal Neoplasms; Genes, ras; Humans; Mutation
Cite
Shinsuke Kazama; Yoichi Ajioka; Hidenobu Watanabe; Toshiaki Watanabe; Hirokazu Nagawa. Not infrequent K-ras mutations in depressed-type early colorectal carcinomas larger than 10 mm. Japanese journal of cancer research : Gann (2002). PMID 11856481. DOI 10.1111/j.1349-7006.2002.tb01256.x.